九龙牦牛肌细胞生成素基因的克隆及其表达谱

doi:10.11843/j.issn.0366-6964.2014.02.006

Abstract

Abstract:

The aim of the present study was to clone myogenin (MyoG) gene of Jiulong yak，to carry out bioinformatics analysis and to assay gene expression profiling in different tissues and developmental stages，in order to lay the foundation for studing meat quality formation and muscle development of Jiulong yak.Based on the published nucleotide sequence of bovine MyoG gene in GenBank，a pair of primer was designed.The Jiulong yak MyoG gene was amplified by RT-PCR，the tissue specificity of MyoG gene were analyzed by semi-quantitative RT-PCR，and different development stages expression specificity of MyoG gene were analyzed by fluorescence quantitative PCR．The results showed that Jiulong yak MyoG gene cDNA was 700 bp (GenBank accession number：KC184120)，with an ORF of 675 bp encoding 224 amino acids.Compared with cattle，human，mouse，rat，pigs，sheep，rabbit，the homology of amino acid sequences were 92%-99%.The MyoG gene expression was only observed in longissimus muscle，and not detected in heart，liver，kidney，spleen and fat.The MyoG mRNA expression level increased with Jiulong yak growth，showing higher mRNA level in longissimus muscle of 3.5-5.5 and over 9 years old Jiulong yaks than that in 0.5 years old Jiulong yaks (P<0.05).These results suggest that MyoG may play an important role in the development of muscle tissue of Jiulong yak.

CLC Number:

LIN Ya-qiu，ZHANG Chang-hui，ZHENG Yu-cai，WANG Yong，HUANG Lin. Cloning and Expression Profiling of Myogenin Gene of Jiulong Yak[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(2): 207-211.

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Cloning and Expression Profiling of Myogenin Gene of Jiulong Yak

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